Mark Ruffalo, Susan Sarandon, Javier Bardem, Cynthia Nixon and Julian Casablancas of The Strokes also contributed, some appearing remotely.
Macklemore’s headline set included tracks such as Thrift Shop, Same Love, Glorious and Can’t Hold Us, alongside his pro-Palestine protest song Hind’s Hall.
Addressing the crowd, the 43-year-old said he had reached a point where staying quiet felt impossible: “It was more uncomfortable for me to stay silent than to say something. I don’t believe that saying something is courageous. I believe that saying something is human.”
The rapper also referenced the unsuccessful effort by 50 members of the House of Lords to persuade Home Secretary Shabana Mahmood to bar him from entering the country, declaring: “Regardless of what the 50 Lords have to say, this is a story of equality for all human beings around this earth.”
The tour was launched after Macklemore was removed from Mr Sheeran’s US Loop tour for making pro-Palestinian remarks on stage. All proceeds from the three European benefit concerts will go to organisations supporting Palestinians.