Luke Hobbs, auctioneer at Gardiner Houlgate, said: “We’re valuing this guitar at around £20,000 to £30,000 based on other recent sales of Johnny Marr’s instruments.
“Marr’s equipment is very popular with collectors and fans.
“Now in his 60s, he’s become something of a national treasure in the UK.”
Other guitars owned by Marr fetched almost £4m at an auction at Christie’s in London in September.
Marr joined The The following a brief period with The Pretenders.
He used the white Fender Stratocaster on stage during The The Versus The World tour which took place in 1989 and 1990.
The guitar can be seen in a film of the tour, also called The The Versus The World.
Recently, Marr has written and recorded with Gorillaz, Billie Eilish, Franz Ferdinand and Noel Gallagher’s High Flying Birds.
The guitar will be available for public viewing at Gardiner Houlgate ahead of the auction. It can be seen by members of the public on 4, 7 and 8 December.