Jo Whittaker, chief executive of Betfred, said: “We have tried hard to protect all our sites and the colleagues who work in them.
“But the combined impact of higher employer national insurance contributions, wage inflation, increases in gambling taxes and wider economic uncertainty has left us with no choice.
“These are well-run shops, staffed by dedicated colleagues, and it is incredibly hard to see any of them close, but the current fiscal and regulatory environment has made it impossible to keep trading all our shops.
“Our priority now is to support the colleagues affected, and to continue serving customers and communities across the rest of our estate.”
Rival gambling operators Paddy Power and William Hill have also shut dozens of stores over the past year.
In January, William Hill and 888 owner Evoke said it moved “quickly and decisively” to offset recent budget changes to gambling taxes through shop closures and cost cuts.
Last October, Paddy Power revealed plans to shut 57 betting shops across the UK and Ireland, in a move which put 250 workers at risk.