Gloucester have been forced into changes to their back line following injuries sustained by Phil Cokanasiga and Ben Redshaw in the win against Exeter.
Cokanasiga had surgery on a foot injury earlier this week and may be out until January, while Redshaw failed a head injury assessment following the Exeter match.
George Barton and Ollie Thorley start at full-back and wing respectively, while Will Joseph shifts inside to centre.
Harlequins are again without fly-half Marcus Smith because of a finger injury, although head coach Jason Gilmore has said the England international should return within the next two weeks.
Prop Fin Baxter (foot) could also be back within the same timeframe, while full-back George Furbank is available again after a head injury and will make his first Prem appearance for Quins since joining from Northampton in the summer.
Gloucester: Barton; Thorley, Joseph, S Atkinson, Loader; C Atkinson, Englefield; Rapava-Ruskin, Lake (c), Fasogbon, Kleyn, Clark, Thomas, Morgan, Trenholm.
Replacements: Innard, Bleuler, C Knight, Clement, Ludlow, Robson, W Knight, Denny.
Harlequins: Furbank; Anderson, Beard, Bradley, Murley (c); Slevin, Porter; Hobson, Walker, Delgado, Dun, Browne, Kenningham, Evans, Carr.
Replacements: Riley, Wenger, Kerrod, Petti, Cunningham-South, Friday, Lang, Green.