Wilkinson’s side opened their Nations League campaign with a 1-0 loss to Italy in Monza, but secured an impressive 1-1 draw with Sweden – who were ranked fifth in the world by Fifa – in Wrexham four days later to earn their first point of the group.
Wilkinson has named an experienced squad with only two uncapped players – Bristol City’s Tianna Teisar and Blackburn Rovers’ Poppy Soper – involved.
The former Canada international says the return of Rowe will help to ensure Wales maintain good standards.
“Rowey is a senior international who has given everything to her country,” she said.
“She’s had a setback from injury, but to have her back in and to bring that confidence, something the players connect with.
“With her at the helm everyone will give everything they have, or they will hear about it. You should have nothing left at the end of playing for your country and she epitomises that.”
Mared Griffiths and Scarlett Hill have been left out of the squad as they will be involved in with Wales Under-19s squad for the Euro round 2 League A qualifiers.
Chelsea midfielder Sophie Ingle remains sidelined due to injury, but will visit the Wales camp.
Wales last faced Denmark in October 2023, losing 2-1 under Gemma Grainger.
They travel to Denmark for their final away Nations League fixture on 30 May before hosting Italy at the Swansea.com Stadium on 3 June ahead of their maiden Euro campaign, which takes place in Switzerland in July.
Wilkinson feels the level of competition remains the ideal preparation for Euro 2025 where Wales will face France, Netherlands and England.
“We know when we get these opportunities we have to make them count,” Wilkinson said.
“Playing these teams we won’t get many chances, we have to be clinical and make sure our possession is in the most dangerous areas.
“We need to be the best version of ourselves.”