Earlier in the evening, Dean Harrison set the fastest time in the solo session.
The Honda Racing rider topped the Superbike leaderboard with a speed of 133.925mph, a time of 16 minutes 54.206 seconds.
The five-time TT winner had earlier posted a lap of 133.222 from a standing start on his opening lap before shaving more than five seconds off his lap time on his second circuit.
The Yorkshireman, now based on the Isle of Man, was a double winner in the Superstock class last year, his first race victories since 2019.
Michael Dunlop was second quickest in the Superbike class on his Hawk Racing Honda at 130.341, almost 28 seconds slower than his rival.
Manx rider Nathan Harrison, Ian Hutchinson, David Johnson and John McGuinness completed the top-six leaderboard.
Australian Josh Brookes led the Superstock qualifying leaderboard thanks to a lap of 130.197 on his DAO Racing Honda, with 14-time winner Peter Hickman second behind the double British Superbike champion with 129.42, followed by Jamie Coward at 128.702.
Dunlop was best of the Supersports on 126.922 on his V2 Ducati, the Northern Ireland rider making a strong start in his bid to extend his run of eight consecutive race wins in the class.
Brookes was second at 124.271 on a Suzuki, then Hickman on his Triumph on 123.584.
Paul Jordan topped the Sportbike speeds thanks to a lap of 120.208 on an Aprilia, with Coward (119.199) and Browne (119.097) second and third respectively.