Bournemouth midfielder Alex Scott has withdrawn from the England squad to face the Czech Republic on Tuesday with a thigh injury.
The 23-year-old is set to be out for six to eight weeks with the quadriceps issue.
Scott was excellent on his full debut as England defeated Croatia 7-0 in the Nations League on Saturday.
He felt the issue prior to the match, but played 70 minutes in central midfield alongside Elliot Anderson before being substituted.
Arsenal defender Ezri Konsa has also been ruled out of the fixture at Wembley, with the 28-year-old to return to the Premier League champions for treatment on an injury.
Bournemouth are due to play nine games between 10 October and 8 November.
Alongside their weekend Premier League commitments, they will play on Wednesday or Thursday for four consecutive weeks, facing Sturm Graz, AC Milan and Sparta Prague in the Europa League and Aston Villa in the fourth round of the Carabao Cup.
England coach Thomas Tuchel was effusive in his praise for Scott after the Croatia match.
“First start and what a start, what a performance,” said Tuchel. “He is so smooth on the ball, his decision making is at the highest level.
“He plays with so much confidence. He is so active, so confident. The work rate off the ball.
“He is just the complete package. He gave us a lot of mobility and a lot of variety and work rate in the centre of the pitch.”
Scott made his England debut in a 2-0 win over the Czech Republic in Prague on Tuesday, before his full debut four days later.
He joins French forward Eli Junior Kroupi on Bournemouth’s injury list, with the 20-year-old still unavailable after sustaining a foot problem in pre-season.
The Cherries are 17th in the Premier League and are winless after five matches, drawing three times and losing twice.
Marco Rose’s side defeated Lincoln to progress in the EFL Cup, and won 2-1 at La Liga side Real Sociedad on their European debut.
They return to Premier League action at Chelsea on Saturday (15:00 BST) and play Sunderland, Manchester United, Leeds and Ipswich prior to the November international break.