Forwards: Sarah Bern (Bristol Bears, 84 caps), Hannah Botterman (Bristol Bears, 62 caps), Delaney Burns (Bristol Bears, 6 caps), Abi Burton (Trailfinders Women, 9 caps), May Campbell (Saracens, 5 caps), Mackenzie Carson (Gloucester-Hartpury, 29 caps), Kelsey Clifford (Saracens, 25 caps), Amy Cokayne (Sale Sharks, 94 caps), Maddie Feaunati (Exeter Chiefs, 27 caps), Bryony Field (Saracens, uncapped), Haidee Head (Trailfinders Women, 1 cap), Lilli Ives Campion (Loughborough Lightning, 8 caps), Hayley Jones (Trailfinders Women, uncapped), Sadia Kabeya, (Loughborough Lightning, 32 caps), Haineala Lutui (Loughborough Lightning, 4 caps), Alex Matthews (Gloucester-Hartpury, 82 caps), Sophie McQueen (Gloucester-Hartpury, uncapped), Maud Muir (Gloucester-Hartpury, 53 caps), Marlie Packer (Harlequins, 116 caps), Demelza Short (Bristol Bears, 4 caps), Morwenna Talling (Sale Sharks, 29 caps)
Backs: Holly Aitchison (Sale Sharks, 49 caps), Jess Breach (Saracens, 57 caps), Millie David (Bristol Bears, 1 cap), Zoe Harrison (Saracens, 70 caps), Tatyana Heard (Sale Sharks, 36 caps), Natasha Hunt (Gloucester-Hartpury, 89 caps), Megan Jones (Trailfinders Women, 38 caps), Ellie Kildunne (Bristol Bears, 62 caps), Claudia Moloney-MacDonald (Exeter Chiefs, 40 caps), Lucy Packer (Harlequins, 44 caps), Flo Robinson (Exeter Chiefs, 5 caps), Helena Rowland (Loughborough Lightning, 51 caps), Emma Sing (Gloucester-Hartpury, 17 caps), Mia Venner (Gloucester-Hartpury, 4 caps), Bo Westcombe-Evans (Trailfinders Women, 2 caps)