Wolves striker Tolu Arokodare forced the cancellation of a training session after he refused to leave the pitch.
BBC Sport has been told the 25-year-old Nigeria international sat down and demanded the club let him move to Fiorentina.
Wolves have rejected a bid from the Serie A side, having accepted an offer from Turkish club Trabzonspor.
Arokodare’s actions led to new boss Cesar Peixoto terminating the session on Monday.
The striker was not involved in Wednesday’s friendly at Maidenhead United as Wolves continue their pre-season plans.
On social media on Wednesday evening, Arokodare wrote: “Throughout my career, I have always taken great pride in conducting myself professionally and maintaining the highest training standards.
“My commitment has always been to prepare myself as well as possible so that I can contribute to my team-mates and the club whenever I am called upon.
“I remain under contract with Wolves, and my only intention is to fulfil my responsibilities professionally by training, doing my job, and contributing to the team in accordance with that contract.”
The latest incident is another flashpoint in the forward’s career at Molineux after he was disciplined in April following a confrontation with young forward Mateus Mane.
Arokodare signed for £24m from Genk last summer but scored just three league goals as Wolves finished bottom of the Premier League and were relegated.
And the incident presents an early challenge for Peixoto just weeks into his reign after he replaced Rob Edwards in a surprise move last month.
The 46-year-old, capped once by Portugal in his playing career, moved from Gil Vicente and will be aiming to return Wolves to the Premier League at the first attempt.